Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats

The study was carried out in Polish goat population to estimate the prevalence of nasal cavity infection with various staphylococcal species including methicillin-resistant Staphylococcus aureus(MRSA), investigate potential permissive role small ruminant lentivirus (SRLV) and determine level clonality S. aureus isolates. Nasal swabs blood samples were collec-ted from 1300 clinically healthy adult goats 21 herds. Blood serological-ly screened for SRLV. Staphylococci isolated identified using classical microbiological methods, MALDI-TOF, multiplex-PCR, their assessed PFGE. Antimicrobial resistance determined on basis minimum inhibitory concentration by demonstration presence mecA gene encoding multiplex-PCR PBP2a protein five main types cassette chromosome mec. apparent staphylococ-cal 29.1% (CI 95%: 26.9%, 31.5%) 7.3% 6.1%, 8.8%), respectively. No relationship found between SRLV-infection any (p=0.143). Only 9.8% isolates resistant amoxicillin/clavulanic acid 5.9% chloramphenicol ciprofloxacin. All tested proved be phenotypically genotypically sensitive methicillin, which yielded appar-ent MRSA 0% 0%, 7.0%). show high genetic similarity within herds, however vary considerably Goats do not appear an important source humans Poland.